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293t cell total protein  (Beijing Solarbio Science)


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    Beijing Solarbio Science 293t cell total protein
    TCF7L2 promotes transcription of PLAUR in GC. a Representative image of the co-localization of TCF7L2 protein (green) and PLAUR (red) in MKN45 cell lines observed by confocal microscopy. b Representative image of the co-localization of TCF7L2 protein (green) and PLAUR (red) in 15 GC tissues (cohort 1) observed via confocal microscopy; the expression of TCF7L2 and PLAUR was positively correlated via fluorescence quantitative analysis. c-d TCF7L2 has a positive regulatory effect on the expression of PLAUR in GC cells. The effects of TCF7L2 silencing on PLAUR mRNA or protein expression in GC cells as detected by RT-qPCR (c) and western blot (d) , respectively. e Motif of the TCF7L2 binding site in the PLAUR promoter, predicted through the JASPAR dataset. f Schematic of the TCF7L2 binding site on the PLAUR promoter. g ChIP-qPCR analysis of TCF7L2 binding to the PLAUR promoter in MKN45 and <t>293T</t> cell lines. The second position was the most important binding site. e Luciferase activity was determined after mutation of the second TCF7L2 site in the PLAUR promoter in MKN45 and 293T cell lines. The luciferase activity of the wild-type PLAUR promoter was significantly higher than that of mutant PLAUR promoter. Compared with the corresponding control group, * P < 0.001.
    293t Cell Total Protein, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/293t+cell+total+protein/pmc09295057-130-8-16?v=Beijing+Solarbio+Science
    Average 93 stars, based on 50 article reviews
    293t cell total protein - by Bioz Stars, 2026-07
    93/100 stars

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    1) Product Images from "TCF7L2 promotes anoikis resistance and metastasis of gastric cancer by transcriptionally activating PLAUR"

    Article Title: TCF7L2 promotes anoikis resistance and metastasis of gastric cancer by transcriptionally activating PLAUR

    Journal: International Journal of Biological Sciences

    doi: 10.7150/ijbs.69933

    TCF7L2 promotes transcription of PLAUR in GC. a Representative image of the co-localization of TCF7L2 protein (green) and PLAUR (red) in MKN45 cell lines observed by confocal microscopy. b Representative image of the co-localization of TCF7L2 protein (green) and PLAUR (red) in 15 GC tissues (cohort 1) observed via confocal microscopy; the expression of TCF7L2 and PLAUR was positively correlated via fluorescence quantitative analysis. c-d TCF7L2 has a positive regulatory effect on the expression of PLAUR in GC cells. The effects of TCF7L2 silencing on PLAUR mRNA or protein expression in GC cells as detected by RT-qPCR (c) and western blot (d) , respectively. e Motif of the TCF7L2 binding site in the PLAUR promoter, predicted through the JASPAR dataset. f Schematic of the TCF7L2 binding site on the PLAUR promoter. g ChIP-qPCR analysis of TCF7L2 binding to the PLAUR promoter in MKN45 and 293T cell lines. The second position was the most important binding site. e Luciferase activity was determined after mutation of the second TCF7L2 site in the PLAUR promoter in MKN45 and 293T cell lines. The luciferase activity of the wild-type PLAUR promoter was significantly higher than that of mutant PLAUR promoter. Compared with the corresponding control group, * P < 0.001.
    Figure Legend Snippet: TCF7L2 promotes transcription of PLAUR in GC. a Representative image of the co-localization of TCF7L2 protein (green) and PLAUR (red) in MKN45 cell lines observed by confocal microscopy. b Representative image of the co-localization of TCF7L2 protein (green) and PLAUR (red) in 15 GC tissues (cohort 1) observed via confocal microscopy; the expression of TCF7L2 and PLAUR was positively correlated via fluorescence quantitative analysis. c-d TCF7L2 has a positive regulatory effect on the expression of PLAUR in GC cells. The effects of TCF7L2 silencing on PLAUR mRNA or protein expression in GC cells as detected by RT-qPCR (c) and western blot (d) , respectively. e Motif of the TCF7L2 binding site in the PLAUR promoter, predicted through the JASPAR dataset. f Schematic of the TCF7L2 binding site on the PLAUR promoter. g ChIP-qPCR analysis of TCF7L2 binding to the PLAUR promoter in MKN45 and 293T cell lines. The second position was the most important binding site. e Luciferase activity was determined after mutation of the second TCF7L2 site in the PLAUR promoter in MKN45 and 293T cell lines. The luciferase activity of the wild-type PLAUR promoter was significantly higher than that of mutant PLAUR promoter. Compared with the corresponding control group, * P < 0.001.

    Techniques Used: Confocal Microscopy, Expressing, Fluorescence, Quantitative RT-PCR, Western Blot, Binding Assay, ChIP-qPCR, Luciferase, Activity Assay, Mutagenesis, Control



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    293t cell total protein  (Beijing Solarbio Science)
    93
    Beijing Solarbio Science 293t cell total protein
    TCF7L2 promotes transcription of PLAUR in GC. a Representative image of the co-localization of TCF7L2 protein (green) and PLAUR (red) in MKN45 cell lines observed by confocal microscopy. b Representative image of the co-localization of TCF7L2 protein (green) and PLAUR (red) in 15 GC tissues (cohort 1) observed via confocal microscopy; the expression of TCF7L2 and PLAUR was positively correlated via fluorescence quantitative analysis. c-d TCF7L2 has a positive regulatory effect on the expression of PLAUR in GC cells. The effects of TCF7L2 silencing on PLAUR mRNA or protein expression in GC cells as detected by RT-qPCR (c) and western blot (d) , respectively. e Motif of the TCF7L2 binding site in the PLAUR promoter, predicted through the JASPAR dataset. f Schematic of the TCF7L2 binding site on the PLAUR promoter. g ChIP-qPCR analysis of TCF7L2 binding to the PLAUR promoter in MKN45 and <t>293T</t> cell lines. The second position was the most important binding site. e Luciferase activity was determined after mutation of the second TCF7L2 site in the PLAUR promoter in MKN45 and 293T cell lines. The luciferase activity of the wild-type PLAUR promoter was significantly higher than that of mutant PLAUR promoter. Compared with the corresponding control group, * P < 0.001.
    293t Cell Total Protein, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/293t+cell+total+protein/pmc09295057-130-8-16?v=Beijing+Solarbio+Science
    Average 93 stars, based on 1 article reviews
    293t cell total protein - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    TCF7L2 promotes transcription of PLAUR in GC. a Representative image of the co-localization of TCF7L2 protein (green) and PLAUR (red) in MKN45 cell lines observed by confocal microscopy. b Representative image of the co-localization of TCF7L2 protein (green) and PLAUR (red) in 15 GC tissues (cohort 1) observed via confocal microscopy; the expression of TCF7L2 and PLAUR was positively correlated via fluorescence quantitative analysis. c-d TCF7L2 has a positive regulatory effect on the expression of PLAUR in GC cells. The effects of TCF7L2 silencing on PLAUR mRNA or protein expression in GC cells as detected by RT-qPCR (c) and western blot (d) , respectively. e Motif of the TCF7L2 binding site in the PLAUR promoter, predicted through the JASPAR dataset. f Schematic of the TCF7L2 binding site on the PLAUR promoter. g ChIP-qPCR analysis of TCF7L2 binding to the PLAUR promoter in MKN45 and 293T cell lines. The second position was the most important binding site. e Luciferase activity was determined after mutation of the second TCF7L2 site in the PLAUR promoter in MKN45 and 293T cell lines. The luciferase activity of the wild-type PLAUR promoter was significantly higher than that of mutant PLAUR promoter. Compared with the corresponding control group, * P < 0.001.

    Journal: International Journal of Biological Sciences

    Article Title: TCF7L2 promotes anoikis resistance and metastasis of gastric cancer by transcriptionally activating PLAUR

    doi: 10.7150/ijbs.69933

    Figure Lengend Snippet: TCF7L2 promotes transcription of PLAUR in GC. a Representative image of the co-localization of TCF7L2 protein (green) and PLAUR (red) in MKN45 cell lines observed by confocal microscopy. b Representative image of the co-localization of TCF7L2 protein (green) and PLAUR (red) in 15 GC tissues (cohort 1) observed via confocal microscopy; the expression of TCF7L2 and PLAUR was positively correlated via fluorescence quantitative analysis. c-d TCF7L2 has a positive regulatory effect on the expression of PLAUR in GC cells. The effects of TCF7L2 silencing on PLAUR mRNA or protein expression in GC cells as detected by RT-qPCR (c) and western blot (d) , respectively. e Motif of the TCF7L2 binding site in the PLAUR promoter, predicted through the JASPAR dataset. f Schematic of the TCF7L2 binding site on the PLAUR promoter. g ChIP-qPCR analysis of TCF7L2 binding to the PLAUR promoter in MKN45 and 293T cell lines. The second position was the most important binding site. e Luciferase activity was determined after mutation of the second TCF7L2 site in the PLAUR promoter in MKN45 and 293T cell lines. The luciferase activity of the wild-type PLAUR promoter was significantly higher than that of mutant PLAUR promoter. Compared with the corresponding control group, * P < 0.001.

    Article Snippet: Lysis buffer was used to extract MKN45 and 293T cell total protein, and a BCA kit (Solarbio, Beijing, China) was used to measure the protein concentration.

    Techniques: Confocal Microscopy, Expressing, Fluorescence, Quantitative RT-PCR, Western Blot, Binding Assay, ChIP-qPCR, Luciferase, Activity Assay, Mutagenesis, Control